RNA Isolation from Mouse Oocytes at The Metaphase II (MII) Stage

Abstract

RNA extraction from oocytes is a fundamental step in various fields of biological research, including developmental biology, reproductive biology, and transcriptomics. The unique molecular characteristics of oocytes make RNA analysis from these cells essential for understanding early developmental processes, reproductive health, evolutionary biology, and disease mechanisms. Below, we highlight the key areas where RNA extraction from oocytes plays a pivotal role, along with the challenges and advancements in this critical procedure.